Coding

Part:BBa_K1122672:Design

Designed by: Aleksandra Lewicka, Jan Lyczakowski   Group: iGEM13_Edinburgh   (2013-08-09)


Alcohol dehydrogenase E (AdhE)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 144
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1212
    Illegal BglII site found at 1419
    Illegal BglII site found at 1695
    Illegal BglII site found at 2373
    Illegal BamHI site found at 862
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 169
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

mutagenesis of two SpeI sites.


Source

gDNA of Weizmann Organism: Clostridium acetobutylicum

References